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Developmentally regulated expression of CGRP in the mouse olfactory pathway
S Denis-Donini1, B Chini, M Vitadello
1CNR Center of Cytopharmacology, Milano, Italy.
The European Journal of Neuroscience
|June 1, 1993
Summary
Calcitonin gene-related peptide (CGRP) expression in mouse olfactory neurons and axons during development suggests its role in olfactory bulb development. This neuropeptide and its mRNA are present from embryonic day 13 until birth.
Area of Science:
- Neuroscience
- Developmental Biology
- Molecular Biology
Background:
- The neuropeptide CGRP plays a role in neuronal development.
- Understanding CGRP's function in the olfactory system is crucial for comprehending olfactory bulb development.
Purpose of the Study:
- To investigate the expression pattern of CGRP and its mRNA in the developing mouse olfactory pathway.
- To determine the role of CGRP as a potential differentiation factor in olfactory bulb ontogenesis.
Main Methods:
- Immunohistochemistry was used to detect the CGRP peptide.
- In situ hybridization identified CGRP mRNA expression.
- Monoclonal antibody against neurofilament subunit NF-M identified olfactory and trigeminal axons.
Main Results:
- CGRP transcripts were first detected at embryonic day 13 (E13), followed by the peptide at E15.
- Both CGRP peptide and mRNA were present until birth, with down-regulated expression postnatally.
- CGRP was observed in specific olfactory receptor neurons after birth.
Conclusions:
- CGRP is expressed in olfactory neurons and their axons during development.
- These findings support CGRP's role as a differentiation factor in olfactory bulb ontogenesis.