A central nervous system keratan sulfate proteoglycan: localization to boundaries in the neonatal rat brain

During the development of the central nervous system (CNS), adhesive molecules promote the formation of axonal pathways and appropriate neuronal connections by facilitating cellular interactions. In addition to the interactions that bring neurons together, recent evidence suggests inhibition of neuronal interactions also plays a role by restricting axons to their appropriate pathways and forming boundaries between functional units of the developing CNS. The present study describes the distribution of a recently identified large keratan sulfate proteoglycan, ABAKAN, in the postnatal day 14 (P14) and adult rat brain. In the adult brain ABAKAN appears to be relatively evenly distributed throughout the CNS, while at P14 this proteoglycan is found at high concentrations between different functional units of the neonatal brain. For example, ABAKAN appears to separate different cortical areas and mark the boundaries between thalamic nuclei. In vitro assays demonstrate that this keratan sulfate proteoglycan is a potent inhibitor of neurite growth. The distribution of ABAKAN at P14 and the effects of this keratan sulfate proteoglycan on neurite growth suggest that ABAKAN functions as a molecular barrier to axonal growth in the developing rat brain.