Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Image processing of electron micrographs for light scattering calculations

D E Freund1, R L McCally, A D Goldfinger

  • 1Applied Physics Laboratory, Johns Hopkins University, Laurel, Maryland 20723-6099.

Cornea
|November 1, 1993
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evaluation of the impact of intrathecal baclofen on the walking ability of people with Multiple Sclerosis related spasticity.

Multiple sclerosis and related disorders·2020
Same author

Point-of-care sonographic diagnosis of maternal small bowel obstruction during pregnancy.

Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology·2016
Same author

Automatic screening of age-related macular degeneration and retinal abnormalities.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference·2012
Same author

Incorporation of an interferon-β neutralizing antibody assay into routine clinical practice.

Multiple sclerosis (Houndmills, Basingstoke, England)·2011
Same author

Humoral immune response to EBV in multiple sclerosis is associated with disease activity on MRI.

Neurology·2009
Same author

Mitomycin C reduces corneal light scattering after excimer keratectomy.

Cornea·2001

A new computer algorithm automates the extraction of corneal stroma fibril data, simplifying light scattering calculations. This method accurately determines fibril positions and radii, crucial for theoretical modeling.

Area of Science:

  • Biophysics
  • Computational Biology
  • Ophthalmology

Background:

  • Theoretical light scattering calculations for corneal stroma models require precise fibril position and radius data.
  • Manual extraction of this structural information from electron micrographs is laborious and time-consuming.
  • Accurate structural data is essential for understanding corneal biomechanics and optical properties.

Purpose of the Study:

  • To develop a computer algorithm for automating the extraction of fibril positions and radii from corneal stroma.
  • To validate the accuracy of the automated method by comparing it with manual measurements.
  • To facilitate theoretical light scattering calculations by providing a more efficient data acquisition process.

Main Methods:

  • Development of a flexible computer algorithm for Macintosh computers.

Related Experiment Videos

  • Automated analysis of electron micrograph data to identify fibril positions and radii.
  • Comparison of light scattering calculations using algorithm-derived data versus manually obtained data.

    • Main Results:

    • The developed computer algorithm successfully automates the extraction of fibril position and radius data.
    • The accuracy of the algorithm's results was validated through comparative analysis.
    • Light scattering calculations using algorithm-derived data closely matched those using manually obtained data.

    Conclusions:

    • The computer algorithm provides a viable and accurate alternative to manual data extraction for corneal stroma.
    • Automation significantly reduces the time and effort required for obtaining essential structural parameters.
    • This tool enhances the feasibility of detailed theoretical light scattering analyses of corneal structures.