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Effect of common protective antigen vaccination to protect mink from challenge exposure with Pseudomonas aeruginosa
Abstract:
The protein portion of endotoxin (OEP), or common antigen of Pseudomonas aeruginosa prepared from strain N10 of P aeruginosa, was examined as a possible vaccine against this organism in mink. Mink were given OEP vaccine and challenge exposed by instillation with the inoculum into the nostril. They survived after this exposure with 4 median lethal (LD50) doses of strain NC-5 (protease negative, elastase negative) or with 160 LD50 doses of strain 5 (protease positive, elastase positive). Strain NC-5 belonged to TYPE 5 AND strain 5 belonged to type 8 (according to the serotyping method of Homma). Strain N10, the source of OEP, belonged to serotype 5. Thus, the mink vaccinated with OEP vaccine were able to stand challenge exposure to both homologous and heterologous serotype strains. More mink given OEP vaccine survived the challenge exposure by intranasal spraying with virulent strain 5 than did those in the nonvaccinated control group, with a significant difference. When the surviving mink of the vaccinated group were rechallenge exposed with 300 or 3,000 LD50 doses of the same strain a month later, most of them survived.
Insights
The outer envelope protein (OEP) vaccine from Pseudomonas aeruginosa protected mink against homologous and heterologous serotypes. Vaccinated mink survived high doses of virulent strains, demonstrating vaccine efficacy.
Area of Science:
- Veterinary immunology
- Microbial pathogenesis
- Vaccine development
Background:
- Pseudomonas aeruginosa is an opportunistic pathogen causing significant infections.
- Developing effective vaccines against P. aeruginosa is crucial for preventing and treating infections.
- The outer envelope protein (OEP) of P. aeruginosa contains conserved antigens with potential vaccine utility.
Purpose of the Study:
- To evaluate the efficacy of an outer envelope protein (OEP) vaccine derived from Pseudomonas aeruginosa strain N10 in protecting mink against challenge exposure.
- To determine if the OEP vaccine provides protection against both homologous and heterologous serotypes of P. aeruginosa.
Main Methods:
- Mink were vaccinated with an OEP vaccine prepared from P. aeruginosa strain N10.
- Vaccinated and control mink were challenge-exposed intranasally with virulent P. aeruginosa strains (NC-5 and strain 5).
- Survival rates and challenge doses (LD50) were recorded, and statistical significance was assessed.
Main Results:
- Mink vaccinated with OEP survived challenge with 4 median lethal doses (LD50) of homologous serotype 5 strain (NC-5) and 160 LD50 of heterologous serotype 8 strain (strain 5).
- A significantly higher survival rate was observed in OEP-vaccinated mink compared to non-vaccinated controls when challenged with virulent strain 5.
- Upon rechallenge with 300 or 3,000 LD50 doses of strain 5 a month later, the majority of surviving vaccinated mink remained protected.
Conclusions:
- The OEP vaccine from P. aeruginosa strain N10 demonstrates significant protective efficacy in mink against challenge with both homologous and heterologous serotypes.
- OEP vaccination provides robust protection, even against high doses of virulent P. aeruginosa strains, suggesting its potential as a broad-spectrum vaccine candidate.
- The study supports the use of conserved outer envelope proteins as a basis for developing effective vaccines against P. aeruginosa infections.