Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Evidence that retroviruses integrate into post-replication host DNA

M Hajihosseini1, L Iavachev, J Price

  • 1National Institute for Medical Research, Mill Hill, London, UK.

The EMBO Journal
|December 15, 1993
PubMed
Summary

Retroviruses integrate into host cell DNA only after it replicates during the S phase. This finding impacts gene therapy and cell lineage studies using retroviral vectors for gene transfer.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Measurement of the charge asymmetry in top quark pair production in <i>pp</i> collisions at [Formula: see text] using the ATLAS detector.

The European physical journal. C, Particles and fields·2015
Same author

Measurement of [Formula: see text] production with a veto on additional central jet activity in <i>pp</i> collisions at [Formula: see text] TeV using the ATLAS detector.

The European physical journal. C, Particles and fields·2015
Same author

Measurement of the top quark mass with the template method in the [Formula: see text] channel using ATLAS data.

The European physical journal. C, Particles and fields·2015
Same author

Search for heavy neutrinos and right-handed <i>W</i> bosons in events with two leptons and jets in <i>pp</i> collisions at [Formula: see text] with the ATLAS detector.

The European physical journal. C, Particles and fields·2015
Same author

Measurement of <i>τ</i> polarization in <i>W</i>→<i>τν</i> decays with the ATLAS detector in <i>pp</i> collisions at [Formula: see text].

The European physical journal. C, Particles and fields·2015
Same author

A search for [Formula: see text] resonances with the ATLAS detector in 2.05 fb<sup>-1</sup> of proton-proton collisions at [Formula: see text].

The European physical journal. C, Particles and fields·2015

Area of Science:

  • Molecular Biology
  • Virology
  • Cell Cycle Biology

Background:

  • Retroviral vectors are widely used for gene transfer in research and therapeutic applications.
  • Understanding the integration timing of retroviral DNA into host cell chromosomes is crucial for optimizing gene delivery strategies.

Purpose of the Study:

  • To determine whether retroviral integration occurs before or after host cell DNA replication during the S phase.
  • To investigate the implications of integration timing for retroviral vector applications.

Main Methods:

  • NIH-3T3 cells were infected with BAG, a replication-incompetent retroviral vector encoding the lacZ gene.
  • Progeny clones from infected single cells were analyzed for the presence of proviral DNA.

Main Results:

Related Experiment Videos

  • Only approximately half of the progeny cells from an infected single cell contained the proviral DNA.
  • This pattern suggests that integration occurs exclusively into post-replication DNA.

Conclusions:

  • Retroviral integration into host chromosomal DNA is a post-replicative event.
  • This finding has significant implications for the efficiency and predictability of gene therapy and cell lineage tracing using retroviral vectors.