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Identification of DNA binding proteins in vaccinia virus by DNA-protein crosslinking
A N Chkheidze1, G G Prikhod'ko, V L Karpov
1W.A. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow.
FEBS Letters
|December 27, 1993
Abstract:
DNA binding proteins of vaccinia virus (VV) virions, strain LIVP, have been studied by their covalent crosslinking to DNA, using two-dimensional gel retardation electrophoresis of crosslinked DNA and proteins as well as the 'protein image' hybridization assay. Five proteins with molecular masses of 16, 25, 27, 41 and 54 kDa, respectively, associated with all analysed DNA sequences, including early and late genes and their promoters, have been identified.