Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

An efficient expression vector for stable expression in human liver cells

D W Kim1, T Harada, I Saito

  • 1Department of Microbiology, Chang Won National University, Korea.

Gene
|December 8, 1993
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evidence for the Collective Nature of Radial Flow in Pb+Pb Collisions with the ATLAS Detector.

Physical review letters·2026
Same author

Evidence for the Dimuon Decay of the Higgs Boson in pp Collisions with the ATLAS Detector.

Physical review letters·2025
Same author

Evidence for Longitudinally Polarized W Bosons in the Electroweak Production of Same-Sign W Boson Pairs in Association with Two Jets in pp Collisions at sqrt[s]=13  TeV with the ATLAS Detector.

Physical review letters·2025
Same author

Observation of tt[over ¯] Production in Pb+Pb Collisions at sqrt[s_{NN}]=5.02  TeV with the ATLAS Detector.

Physical review letters·2025
Same author

Search for Dark Matter Produced in Association with a Dark Higgs Boson in the bb[over ¯] Final State Using pp Collisions at sqrt[s]=13  TeV with the ATLAS Detector.

Physical review letters·2025
Same author

Search for Magnetic Monopole Pair Production in Ultraperipheral Pb+Pb Collisions at sqrt[s_{NN}]=5.36  TeV with the ATLAS Detector at the LHC.

Physical review letters·2025
Same journal

Mutation T71R enhanced the structural stability and functional activity of wild type superoxide dismutase cloned from soil metagenome.

Gene·2026
Same journal

Reduced ATXN1 expression as an adverse prognostic indicator in Acute myeloid leukemia.

Gene·2026
Same journal

Constructing regulatory networks of Rubisco post-translational modifications: a novel avenue for engineering environment adaptive plants.

Gene·2026
Same journal

Traumatic brain injury enhances fracture healing by upregulating VNN1 to activate the Wnt/β-catenin signaling pathway.

Gene·2026
Same journal

Single-cell transcriptomics reveals CCL2-mediated macrophage-endothelial cell interactions drive apoptosis in varicose veins.

Gene·2026
Same journal

Development of a gene signature related to phospholipid metabolism for prognosis and therapeutic Prediction in Osteosarcoma: Focus on VAC14.

Gene·2026
See all related articles

The human elongation factor 1 alpha (EF-1 alpha) promoter drives strong neomycin resistance (NmR) gene expression in liver cells. This EF-1 alpha promoter shows superior performance compared to other common promoters for gene expression studies.

Area of Science:

  • Molecular Biology
  • Gene Expression
  • Biotechnology

Background:

  • Investigating gene expression control is crucial for biotechnology.
  • Promoters regulate gene transcription, influencing protein production.
  • Identifying strong promoters enhances gene delivery and expression systems.

Purpose of the Study:

  • To evaluate the human polypeptide chain elongation factor 1 alpha (EF-1 alpha) promoter for driving bacterial neomycin resistance (NmR) gene expression.
  • To compare the efficacy of the EF-1 alpha promoter against other commonly used promoters in a human liver cell line.

Main Methods:

  • Utilized the plasmid pEF321-neo containing the NmR gene under the EF-1 alpha promoter.
  • Transfected the human liver cell line HepG2.
  • Compared NmR gene expression levels with vectors using SV40 early region, thymidine kinase, and enhancer-linked promoters.

Related Experiment Videos

Main Results:

  • The EF-1 alpha promoter (in pEF321-neo) demonstrated significantly higher NmR gene expression in HepG2 cells.
  • Expression levels surpassed those driven by SV40 early region and thymidine kinase promoters.
  • The EF-1 alpha promoter outperformed other tested promoter constructs.

Conclusions:

  • The EF-1 alpha promoter is a highly effective tool for driving high-level gene expression in human liver cells.
  • This finding has implications for developing advanced gene expression vectors and biotechnological applications.
  • The EF-1 alpha promoter offers a robust alternative for gene expression studies requiring strong transcriptional activity.