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Updated: Aug 22, 2026

Detecting the Lyme Disease Spirochete, Borrelia Burgdorferi, in Ticks Using Nested PCR
Published on: February 4, 2018
[Clinico-laboratory diagnosis of Lyme borreliosis--personal experience]
Abstract:
Between May 1992 and February (second half) 1993 two hundred patients with the history of tick bite have been examined at the Institute for Infectious and Tropical Diseases "Dr Kosta Todorović". Of the above 200 persons, 103 of them have had clinical/epidemiological evidence of Lyme disease (i.e. Erythema migrans). In order to establish the degree of seroconversion in this stage of Lyme disease, blood of each patient has been tested by PHA and IFA methods. Blood from each patient has been drawn in pairs, immediately before and after the treatment. By method of PHA we have analyzed 186 sera and found 23 positive results. By method of IFA we have analyzed 10 sera and found one positive result. By Comparative testing we have analyzed 10 sera, obtaining 5 positive results by PHA and 3 positive results ba IFA.
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