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Related Experiment Videos

Basic fibroblast growth factor dilates rat pial arterioles

S Rosenblatt1, K Irikura, C G Caday

  • 1Stroke Research Laboratory, Massachusetts General Hospital, Boston 02114.

Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism
|January 1, 1994
PubMed
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Basic fibroblast growth factor (bFGF) causes dose-dependent vasodilation in rat pial arterioles, mediated by nitric oxide (NO). This suggests bFGF regulates cerebrovascular tone and cerebral blood flow.

Area of Science:

  • Neuroscience
  • Cardiovascular Physiology
  • Molecular Biology

Background:

  • Basic fibroblast growth factor (bFGF) is a polypeptide influencing neuronal, glial, and endothelial cell survival and differentiation.
  • Systemic administration of bFGF has been shown to lower blood pressure via vasodilation, partly through nitric oxide (NO)-dependent pathways.

Purpose of the Study:

  • To directly investigate the effect of bFGF on pial arterioles in vivo.
  • To elucidate the role of nitric oxide (NO) in bFGF-mediated cerebrovascular effects.

Main Methods:

  • Utilized the closed cranial window technique in pentobarbital-anesthetized Sprague-Dawley rats (n=18).
  • Administered varying concentrations of bFGF (5-200 ng/ml) and assessed pial arteriole diameter.
  • Investigated the involvement of NO by using the NO synthase inhibitor NG-nitro-L-arginine methyl ester (L-NAME) and heat-inactivated bFGF or blocking antibodies.

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Main Results:

  • Basic FGF induced a dose-dependent vasodilation, with maximal effect at 100 ng/ml (approx. 120% of control).
  • No vasodilation was observed with heat-inactivated bFGF or bFGF preincubated with a blocking antibody.
  • bFGF-induced vasodilation was significantly attenuated by L-NAME, confirming an NO-dependent mechanism.

Conclusions:

  • Basic FGF directly causes vasodilation of pial arterioles.
  • The vasodilatory effect of bFGF on cerebral vessels is mediated, at least in part, by nitric oxide.
  • bFGF may play a significant role in regulating cerebrovascular tone and cerebral blood flow.