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Detection of a shared idiotope on two encephalomyocarditis virus neutralizing monoclonal antibodies by neutralization
C A Kraaijeveld1, T A Oosterlaken, F Vlaspolder
1Eijkman-Winkler Laboratory for Medical Microbiology, Utrecht University, The Netherlands.
Journal of Virological Methods
|October 1, 1993
Abstract:
Idiotypic cross-reactivity between encephalomyocarditis virus (EMCV) neutralizing monoclonal antibodies UM 21.1 (IgG2b) and UM 21.3 (IgG2a) was detected by neutralization inhibition enzyme immunoassay using polyclonal and monoclonal anti-idiotypic antibodies. One strongly cross-reactive anti-idiotypic monoclonal antibody, designated 21.1A5 (IgG2b), might recognize a recurrent idiotope on EMCV neutralizing antibodies but it did not induce EMCV neutralizing anti-anti-idiotypic antibodies in homologous BALB/c mice.