Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Structural features of the 26 S proteasome complex

J M Peters1, Z Cejka, J R Harris

  • 1Division of Cell Biology, German Cancer Research Center, Heidelberg.

Journal of Molecular Biology
|December 20, 1993
PubMed
Summary

The 26S proteasome structure reveals regulatory subunits attached to the 20S core, indicating precise subunit positioning essential for protein degradation and antigen processing.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Recurrence quantification analysis of resting state EEG signals in autism spectrum disorder - a systematic methodological exploration of technical and demographic confounders in the search for biomarkers.

BMC medicine·2018
Same author

Neurophysiological evidence of preserved connectivity in tuber tissue.

Epilepsy & behavior case reports·2017
Same author

Treatment of multifocal breast cancer by systemic delivery of dual-targeted adeno-associated viral vectors.

Gene therapy·2015
Same author

COMBINED DELAY AND GRAPH EMBEDDING OF EPILEPTIC DISCHARGES IN EEG REVEALS COMPLEX AND RECURRENT NONLINEAR DYNAMICS.

Proceedings. IEEE International Symposium on Biomedical Imaging·2015
Same author

Treatment of multifocal breast cancer by systemic delivery of dual-targeted adeno-associated viral vectors.

Gene therapy·2015
Same author

PPARβ/δ promotes HRAS-induced senescence and tumor suppression by potentiating p-ERK and repressing p-AKT signaling.

Oncogene·2013

Area of Science:

  • Molecular Biology
  • Structural Biology
  • Cellular Biology

Background:

  • Proteasomes are crucial for degrading abnormal and regulatory proteins, and for antigen processing.
  • The 20S proteasome is the proteolytic core of the larger 26S proteasome complex.
  • The 26S proteasome includes additional subunits involved in regulation and substrate recognition.

Purpose of the Study:

  • To elucidate the structural organization of the 26S proteasome complex.
  • To investigate the arrangement of regulatory subunits relative to the 20S core.
  • To understand the implications of the complex's structure for subunit positioning.

Main Methods:

  • Electron microscopy was employed to visualize the 26S proteasome.
  • Digital image analysis was used to process and interpret the structural data.

Related Experiment Videos

Main Results:

  • The 26S proteasome has an estimated molecular weight of approximately 2000 kDa.
  • Two asymmetric masses, likely regulatory subunits, attach to both ends of the dimeric 20S proteasome.
  • The complex exhibits high structural uniformity, suggesting precise subunit arrangement.

Conclusions:

  • The structure of the 26S proteasome reveals a defined organization with regulatory components.
  • The precise positioning of alpha-type and beta-type subunits within the 20S proteasome is confirmed.
  • This structural insight is vital for understanding proteasome function in protein homeostasis and immune response.