Active heterologous chain recombinants of monoclonal antibodies raised in related rabbits

Heterologous chain recombinants of homogeneous anti-streptococcal group A-variant polysaccharide antibodies produced by pedigreed rabbits regain in certain pairs the same antigen-binding capacity as the homologous pairs. In contrast, chain recombinants with antibodies from nonrelated rabbits are much less active. This data suggests random pairing of immunoglobulin heavy and light chains coded for in the germ line and subsequent selection.