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Expression of Lyn protein on human malignant lymphomas
S H Choi1, Y Yamanashi, M Shiota
1Department of Pathology and Oncology, University of Tokyo.
Summary
Lyn protein expression in human malignant lymphomas (MLs) generally mirrors normal cells but can be down-regulated in Epstein-Barr virus-transformed MLs, particularly in immunocompromised hosts.
Area of Science:
- Oncology
- Molecular Biology
- Immunology
Background:
- Lyn is a src family tyrosine kinase expressed in B lymphocytes and monocytes/macrophages.
- Lyn protein is involved in B lymphocyte signal transduction, potentially via association with membrane-bound IgM.
Purpose of the Study:
- To investigate Lyn protein expression in human malignant lymphomas (MLs).
- To analyze Lyn expression patterns in various ML subtypes and under different conditions.
Main Methods:
- Immunohistology, immunochemistry, and Southern blot analysis were used.
- Studied 50 ML biopsies, 12 SCID mouse-maintained ML samples, and 4 African Burkitt cell lines.
Main Results:
- Lyn was expressed in most B-MLs (27/27) but less frequently in T-MLs (5/21) and null-MLs (2/2).
- Epstein-Barr virus-transformed B cells in SCID mice showed down-regulated Lyn expression.
- Lyn expression was decreased in some Epstein-Barr virus-positive African Burkitt's ML lines.
- Southern blot analysis indicated no changes in the lyn gene itself.
Conclusions:
- Lyn expression in MLs generally reflects normal counterparts.
- Epstein-Barr virus-transformed MLs in immunocompromised hosts may exhibit altered Lyn expression, often down-regulated.
- This study is the first to report Lyn expression on human MLs.