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Formate dehydrogenase activity in methanol-utilizing yeasts
V Illeová1, M Certík, V Stefuca
1Department of Chemical Engineering, Faculty of Chemical Technology, Slovak Technical University, Bratislava.
Microbios
|January 1, 1993
Summary
Formate dehydrogenase (FDH) activity in methanol-utilizing yeasts is inhibited at 4% methanol. Pichia lindneri showed the highest FDH activity, while Candida boidinii and Pichia trehalophila exhibited none.
Area of Science:
- Biochemistry
- Microbiology
- Enzymology
Background:
- Methanol-utilizing yeasts are crucial for biotechnological applications.
- Formate dehydrogenase (FDH) is a key enzyme in methanol metabolism.
- Understanding FDH activity under varying methanol concentrations is essential for optimizing yeast fermentation.
Purpose of the Study:
- To investigate the growth and formate dehydrogenase (FDH) activity in five different methanol-utilizing yeasts.
- To determine the optimal methanol concentration for FDH activity.
- To identify yeast strains with high FDH potential.
Main Methods:
- Culturing five species of methanol-utilizing yeasts.
- Assessing yeast growth at different methanol concentrations (0.5% to 4%).
- Measuring formate dehydrogenase (FDH) activity using spectrophotometric assays.
Main Results:
- Yeast growth and FDH activity were generally inhibited at 4% methanol concentration.
- Candida boidinii and Pichia trehalophila showed no detectable FDH activity.
- Pichia lindneri exhibited the highest FDH activity, reaching 0.14 U/mg protein.
Conclusions:
- Methanol concentration significantly impacts FDH activity in yeasts.
- Pichia lindneri is a promising candidate for applications requiring high FDH activity.
- Further research is needed to explore FDH potential in other methanol-utilizing yeasts.