Amyotrophic lateral sclerosis: quantitative autoradiography of [3H]MK-801/NMDA binding sites in spinal cord

We have characterized a high-affinity binding site for [3H]MK-801, an NMDA receptor ion channel antagonist, in cervical spinal cords from patients who have died with amyotrophic lateral sclerosis (ALS) and from control subjects. In cervical spinal cord [3H]MK-801 labelled at least two binding sites, the highest affinity site having a Kd of between 9-16 nM. No significant differences in affinity were observed between spinal cords from ALS patients or controls. In spinal cords from ALS patients, large reductions in [3H]MK-801 receptor binding (between 30-40% reductions) were detected in both the dorsal and ventral horns. These data may reflect the death of receptor-bearing cells, or a form of receptor regulation.