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Analysis of Cell Cycle Position in Mammalian Cells
Published on: January 21, 2012
Correlation of (S+G2)-phase fractions determined by flow cytometry and Ki-67 labelling indices in colorectal
F Zölzer1, K Gilbertz, C Streffer
1Institut für Medizinische Strahlenbiologie, Universitätsklinikum Essen, BRD.
Abstract:
Samples from 14 human colorectal carcinomas were analysed by means of classical (one-parameter) flow cytometry. (S+G2)-phase fractions were calculated from DNA histograms with the help of two different algorithms. At the same time, the percentage of cells positive for the Ki-67 antibody, which recognizes proliferating cells, was determined in cytocentrifuge preparations. Significant correlations between the two indices were found only if the histogram analysis included background subtraction and aggregate correction. This finding may throw some light on the reliability of prognosis based on flow cytometry.
