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Rabbit corneal endothelial cell membrane potential

C G Wigham1, K Green, S Hodson

  • 1Department of Optometry and Vision Sciences, University of Wales College of Cardiff, UK.

Ophthalmic & Physiological Optics : the Journal of the British College of Ophthalmic Opticians (Optometrists)
|July 1, 1993
PubMed
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Rabbit corneal endothelial cells exhibit a membrane potential of -29.3 mV, indicating cell coupling and active ion transport. This study reveals key insights into the electrophysiology of these vital cells.

Area of Science:

  • Ophthalmology
  • Cell Physiology
  • Membrane Biophysics

Background:

  • Rabbit corneal endothelial cells form a critical barrier in the eye.
  • Understanding their electrical properties is essential for ocular health.

Purpose of the Study:

  • To measure the membrane potential of rabbit corneal endothelial cells.
  • To investigate cell-to-cell communication (coupling) within the endothelium.
  • To elucidate the mechanisms generating transmembrane electrolyte gradients.

Main Methods:

  • Intracellular microelectrode impalement to measure membrane potential.
  • Iontophoresis of Lucifer Yellow dye to confirm electrode placement and assess cell coupling.
  • Pharmacological manipulation using ouabain and high extracellular potassium to probe membrane responsiveness and ion permeability.

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Main Results:

  • The average membrane potential was measured at -29.3 +/- 0.8 mV.
  • Lucifer Yellow diffusion confirmed intercellular coupling, extending five to six cell diameters.
  • Cells responded to ouabain and high potassium, demonstrating selective permeability to potassium and active Na(+)-K(+)-ATPase activity.

Conclusions:

  • Rabbit corneal endothelial cells possess a negative resting membrane potential.
  • Significant electrical coupling exists between these endothelial cells.
  • Na(+)-K(+)-ATPase activity is crucial for maintaining the electrolyte gradients across the corneal endothelium.