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Elimination of aminoglycoside antibiotics in milk following intramammary administration
1Ministère de l'Agriculture, Centre National d'Etudes et Alimentaires, Laboratoire des Médicaments Vétérinaires, Fougères, France.
Abstract:
The elimination in cow milk of aminoglycoside antibiotic residues (neomycin, dihydrostreptomycin, kanamycin and gentamicin) was studied after intramammary administration of eight drug formulations marketed in France. Quantitative residue analysis was performed by a cylinder plate method. The sensitivity was 0.15 microgram/ml for neomycin, dihydrostreptomycin, kanamycin and 0.025 microgram/ml for gentamicin. The mean elimination periods ranged between 4 and 13 milkings. Several ways of assessing withdrawal times are discussed.
Insights
Cow milk antibiotic residue elimination was studied for four aminoglycosides. Mean elimination periods ranged from 4 to 13 milkings, informing safe withdrawal times.
Area of Science:
- Veterinary Pharmacology
- Food Safety
- Dairy Science
Background:
- Aminoglycoside antibiotics are used to treat bovine mastitis.
- Residues of these antibiotics in cow milk pose a food safety concern.
- Understanding elimination kinetics is crucial for establishing withdrawal periods.
Purpose of the Study:
- To determine the elimination periods of neomycin, dihydrostreptomycin, kanamycin, and gentamicin from cow milk after intramammary administration.
- To evaluate eight different drug formulations available in France.
- To discuss methods for assessing appropriate withdrawal times.
Main Methods:
- Intramammary administration of eight aminoglycoside formulations to cows.
- Quantitative residue analysis of milk samples using a cylinder plate method.
- Determination of detection limits: 0.15 µg/mL for neomycin, dihydrostreptomycin, kanamycin; 0.025 µg/mL for gentamicin.
Main Results:
- Mean elimination periods varied between 4 and 13 milkings across the studied antibiotics and formulations.
- The cylinder plate method provided sensitive detection of aminoglycoside residues.
- Variability in elimination times suggests formulation-dependent differences.
Conclusions:
- Established elimination periods provide a basis for setting withdrawal times to ensure milk safety.
- Further research into factors influencing aminoglycoside elimination may refine withdrawal time recommendations.
- Monitoring antibiotic residues in milk is essential for public health and regulatory compliance.