Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

DNA histogram typing in human seminomas

A Zlotta1, R Kiss, R Vanvelthoven

  • 1Department of Urology, Erasme Hospital, University Clinics of Brussels, Belgium.

Anticancer Research
|September 1, 1993
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Reply by Authors.

The Journal of urology·2025
Same author

Characterizing the Impact of Novel Patient-Centered Pathology Reports on Men Undergoing Prostate Biopsy: The Patient-Centered Pathology Report Randomized Controlled Trial.

The Journal of urology·2025
Same author

Reply by Authors.

The Journal of urology·2020
Same author

Long-term use of 5-alpha-reductase inhibitors is safe and effective in men on active surveillance for prostate cancer.

Prostate cancer and prostatic diseases·2020
Same author

The Prostate Cancer Rehabilitation Clinic: a biopsychosocial clinic for sexual dysfunction after radical prostatectomy.

Current oncology (Toronto, Ont.)·2019
Same author

[Analysis of positive margins risk factors and long term recurrence in the conservative treatment of breast cancer].

Revue medicale de Bruxelles·2017

Normal testicular tissues show varied DNA content, while seminomas exhibit abnormal DNA patterns. Further research is needed to determine the prognostic value of DNA histograms in seminoma grading.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Seminoma is a common testicular germ cell tumor.
  • Accurate characterization of tumor cell DNA content is crucial for understanding cancer biology and prognosis.
  • Previous studies on DNA content in normal and neoplastic testicular tissues have yielded varied results.

Purpose of the Study:

  • To characterize the nuclear DNA content and proliferative activity in normal testicular tissues and seminomas.
  • To investigate the potential of DNA histogram analysis for seminoma diagnosis and grading.

Main Methods:

  • Feulgen staining of formalin-fixed paraffin-embedded testicular tissues.
  • Cell image processing to compute integrated optical density of Feulgen-stained nuclei.
  • Analysis of DNA histograms to identify cell nuclei populations (haploid, diploid, tetraploid).

Related Experiment Videos

  • Determination of proliferative activity in seminoma samples.

    • Main Results:

    • Normal testicular tissues displayed DNA histograms with three distinct cell nuclei populations (haploid, diploid, tetraploid) in varying proportions.
    • Most seminomas exhibited monomorphic DNA histogram patterns.
    • Seminomas showed a predominance of G0-G1 phase cell nuclei with abnormal DNA content (3c-4c range).

    Conclusions:

    • Nuclear DNA content analysis reveals distinct patterns in normal testicular tissue versus seminoma.
    • Seminomas exhibit aberrant DNA content and cell cycle distribution.
    • Further investigation is required to establish the prognostic and grading value of DNA histograms in seminoma.