Related Experiment Video

Updated: Aug 14, 2026

Use of Fluorescent Immuno-Chemistry for the detection of Edwardsiella ictaluri in channel catfish (I. punctatus) samples
05:23

Use of Fluorescent Immuno-Chemistry for the detection of Edwardsiella ictaluri in channel catfish (I. punctatus) samples

Published on: May 10, 2011

Crossed immunoelectrophoresis used for bacteriological diagnosis in patients with endocarditis

A Kjerulf1, M Tvede, N Høiby

  • 1Department of Clinical Microbiology, Rigshospitalet, Copenhagen, Denmark.

APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica
|October 1, 1993
PubMed

Insights

Crossed immunoelectrophoresis effectively detects antibodies for viridans streptococci endocarditis, offering high diagnostic sensitivity. This method is less reliable for identifying other bacterial causes of endocarditis.

Area of Science:

  • Medical Microbiology
  • Immunology
  • Infectious Diseases

Background:

  • Endocarditis is a serious infection of the heart valves.
  • Accurate etiological diagnosis is crucial for effective treatment.
  • Traditional diagnostic methods can be challenging, especially for culture-negative cases.

Purpose of the Study:

  • To evaluate the utility of crossed immunoelectrophoresis (CIE) for detecting antibodies against endocarditis-causing bacteria.
  • To assess the diagnostic performance of CIE in patients with suspected endocarditis.

Main Methods:

  • Sera from 151 patients with suspected endocarditis were analyzed.
  • Crossed immunoelectrophoresis was employed to detect precipitating antibodies.
  • Patients were categorized into definite, culture-negative, uncertain, and no endocarditis groups.

Main Results:

  • CIE demonstrated high diagnostic specificity (86%) and sensitivity (100%) for endocarditis caused by viridans streptococci.
  • The method showed lower reliability in identifying other bacterial etiologies of endocarditis.
  • Precipitating antibodies were clearly identified in patients with viridans streptococci endocarditis.

Conclusions:

  • Crossed immunoelectrophoresis is a valuable tool for diagnosing viridans streptococci endocarditis.
  • Further development of CIE assays may be needed for broader bacterial coverage in endocarditis diagnosis.

More Related Videos

Simultaneous Detection of Different Antibody Classes in a Multiplexed Serological Test05:25

Simultaneous Detection of Different Antibody Classes in a Multiplexed Serological Test

Published on: July 14, 2023

Direct Microbial Identification using An Automated Microbial Identification System to Facilitate the EUCAST RAST Method Without Mass Spectrometry09:07

Direct Microbial Identification using An Automated Microbial Identification System to Facilitate the EUCAST RAST Method Without Mass Spectrometry

Published on: May 24, 2024

Related Concept Videos

Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
Myocarditis II: Clinical Features and Diagnostic Tests01:27

Myocarditis II: Clinical Features and Diagnostic Tests

Myocarditis is an inflammation of the heart muscle. The symptoms vary widely, encompassing asymptomatic presentations to severe, acute manifestations.Clinical PresentationAsymptomatic cases: In some instances, myocarditis may be asymptomatic, with the infection resolving without intervention. These cases often go undetected unless discovered incidentally through diagnostic imaging or tests conducted for other reasons.General Early Symptoms: Early symptoms of myocarditis are non-specific and can...
Rapid Identification of Pathogens01:25

Rapid Identification of Pathogens

MALDI-TOF MS has transformed clinical microbiology by offering a rapid and reliable method for pathogen identification. The traditional approach to microbial identification typically involves time-consuming culture techniques and biochemical tests, which can delay the initiation of appropriate antimicrobial therapy. MALDI-TOF MS avoids these delays by using characteristic ribosomal protein mass patterns of microbial cells, enabling accurate species-level identification within minutes.Principle...
Automated Microbial Diagnostics01:24

Automated Microbial Diagnostics

Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...