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Immunocytochemical detection of bromodeoxyuridine in tissues of the toad Bufo arenarum Hensel
J J Gagliardino1, C L Gómez Dumm, C E Bianchi
1Center of Experimental and Applied Endocrinology, School of Medical Sciences, National University of La Plata, Argentina.
Abstract:
We present a modified protocol for the immunocytochemical identification of 5-bromo-2'-deoxyuridine (BrdU) as an indicator of cell replication in different tissues of the toad, Bufo arenarum Hensel. Animals were sacrificed 60 min after BrdU (5 mg/100 g body weight) was injected into the dorsal lymph sac. The tissues were fixed in Carnoy's fluid and stained by the immunoperoxidase method using an anti-BrdU monoclonal antibody. This protocol can be used safely for the study of cell replication in toads and other Anura amphibia.