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Robust 3D DNA FISH Using Directly Labeled Probes
Published on: August 15, 2013
The genetic map around the tail kinks (tk) locus on mouse chromosome 9
Summary
Researchers mapped the tail kinks (tk) gene on mouse Chromosome 9. They identified the D9Mit9 microsatellite marker as a key starting point for cloning the gene responsible for this skeletal mutation.
Area of Science:
- Genetics
- Genomics
- Mouse models
Background:
- Tail kinks (tk) is a classical mouse skeletal mutation.
- Understanding the genetic basis of skeletal mutations is crucial for developmental biology.
Purpose of the Study:
- To establish a genetic map of the region surrounding the tk locus on mouse Chromosome 9.
- To identify markers for positional cloning of the tk gene.
Main Methods:
- Generated a backcross segregating for tk.
- Analyzed 1004 progeny for coat-color phenotype (dilute gene, d) and microsatellite marker D9Mit12.
- Mapped five microsatellite loci, d, and Mod-1 relative to tk.
Main Results:
- Identified 56 recombinants between d and tk, and 75 between tk and D9Mit12.
- Established a panel of 130 recombinants, including one double recombinant.
- Found the D9Mit9 microsatellite marker showed no recombination with tk.
Conclusions:
- The D9Mit9 locus is tightly linked to the tk gene.
- D9Mit9 is a suitable starting point for a chromosomal walk to isolate the tk gene.
