Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Quantitative enzyme cytochemistry during human macrophage development

R J Sokol1, G Hudson, J M Wales

  • 1Department of Medicine, University of Sheffield, UK.

Journal of Anatomy
|August 1, 1993
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Investigating a psychological model of mental conditions and coping during the COVID-19 pandemic driven by participatory methods.

Social psychiatry and psychiatric epidemiology·2022
Same author

Neuropathological and biochemical investigation of Hereditary Ferritinopathy cases with ferritin light chain mutation: Prominent protein aggregation in the absence of major mitochondrial or oxidative stress.

Neuropathology and applied neurobiology·2020
Same author

Exome sequencing in dementia with Lewy bodies.

Translational psychiatry·2016
Same author

Extraction Time of Kidneys From Deceased Donors and Impact on Outcomes.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2015
Same author

Loss of pediatric kidney grafts during the "high-risk age window": insights from pediatric liver and simultaneous liver-kidney recipients.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2015
Same author

Left ventricular assist device exchange for persistent infection: a case series and review of the literature.

Transplant infectious disease : an official journal of the Transplantation Society·2014
Same journal

Skin denticle diversity in the giant manta and the lesser devil ray: A three-dimensional analysis.

Journal of anatomy·2026
Same journal

Two-step workflow integrating automatic registration and manual refinement for the accurate alignment of serial histological sections in 3D reconstruction.

Journal of anatomy·2026
Same journal

Correction to "Cajal-Retzius neurons are required for the development of the human hippocampal fissure".

Journal of anatomy·2026
Same journal

Loss of primary cilia in late pituitary organogenesis does not cause endocrine dysfunction.

Journal of anatomy·2026
Same journal

Cumulative effects of lifelong systemic excess growth hormone on postcranial skeletal morphology in adult mice.

Journal of anatomy·2026
Same journal

Layer-by-layer soft-tissue effects on flexion-extension-dominant passive ex vivo limb joint ROM in quadrupedal mammals: An anatomical contribution to a morphofunctional framework.

Journal of anatomy·2026
See all related articles

This study tracked enzyme activity in developing human macrophages, finding significant increases in NADH dehydrogenase, succinate dehydrogenase, acid phosphatase, and alpha-naphthyl butyrate esterase over time. These changes reflect enhanced macrophage function during development.

Area of Science:

  • Cell Biology
  • Immunology
  • Biochemistry

Background:

  • Macrophages are crucial immune cells involved in phagocytosis and inflammatory responses.
  • Understanding macrophage development and functional maturation is key to immunology.
  • Enzyme activity is a key indicator of cellular metabolic state and function.

Purpose of the Study:

  • To investigate changes in intracellular enzyme activity during human monocyte-derived macrophage development.
  • To quantify the activity of four specific enzymes: NADH dehydrogenase, succinate dehydrogenase, acid phosphatase, and alpha-naphthyl butyrate esterase.
  • To correlate enzyme activity with macrophage maturation and functional capacity.

Main Methods:

  • Human peripheral blood monocytes were cultured for 6 days.

Related Experiment Videos

  • Mononuclear phagocytes were isolated using glass adherence.
  • Cytochemical staining was performed for four key enzymes.
  • Microdensitometry was employed to measure staining intensity and enzyme activity in individual cells.

    • Main Results:

    • A statistically significant increase in the activity of all four enzymes (NADH dehydrogenase, succinate dehydrogenase, acid phosphatase, alpha-naphthyl butyrate esterase) was observed with increasing culture time.
    • Enzyme activity increased progressively from day 0 to day 6 of macrophage development.
    • The observed increases suggest enhanced mitochondrial, lysosomal, and ectoenzyme functions.

    Conclusions:

    • Macrophage development is characterized by a substantial and progressive increase in the activity of key intracellular enzymes.
    • These enzymatic changes signify enhanced functional capabilities, including metabolic and degradative processes, as macrophages mature.
    • The findings provide quantitative insights into the biochemical maturation of human macrophages.