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Microsecond response of a voltage-sensitive merocyanine dye: fast voltage-clamp measurements on squid giant axon
B M Salzberg1, A L Obaid, F Bezanilla
1Department of Neuroscience, University of Pennsylvania School of Medicine, Philadelphia 19104-6074.
Abstract:
Extrinsic absorption changes exhibited by squid giant axons stained with the voltage-sensitive merocyanine-oxazolone dye NK 2367 were measured during brief voltage-clamp steps. Experiments that employed an optical recording system having a sub-microsecond response time constant demonstrate that this dye responds to step changes in membrane voltage in less than 2 microseconds at room temperature. The optical response is independent of ionic currents and is unlikely to depend upon cytoskeletal or cytoplasmic events.
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