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Related Experiment Videos

beta 2-Microglobulin and granulocyte elastase

B Wehle1, J Bergstrom, T Kishimoto

  • 1Department of Renal Medicine, Karolinska Institute, Huddinge University Hospital, Stockholm, Sweden.

Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association
|January 1, 1993
PubMed
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This study evaluated beta 2-microglobulin (B2M) and elastase levels during dialysis. Conventional dialyzers did not alter B2M levels, while high-permeability membranes reduced them, indicating B2M elimination. All dialyzers increased elastase, with cuprammonium cellulose showing the highest rise.

Area of Science:

  • Nephrology
  • Biocompatibility
  • Biochemistry

Background:

  • Plasma beta 2-microglobulin (B2M) is a marker of kidney function and inflammation.
  • Dialysis involves extracorporeal blood circulation, potentially affecting B2M and inflammatory markers.

Purpose of the Study:

  • To assess the impact of different dialyzer membranes on plasma B2M levels.
  • To evaluate the generation of granulocyte elastase-alpha 1-antiproteinase complex during dialysis with various dialyzers.

Main Methods:

  • Radioimmunoassay was used to measure plasma B2M before and after dialysis with seven different dialyzers.
  • Plasma B2M was corrected for extracellular fluid volume changes.
  • Granulocyte elastase-alpha 1-antiproteinase complex was measured in arterial blood samples during dialysis.

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Main Results:

  • Conventional dialyzers showed no significant change in corrected plasma B2M levels.
  • High-permeability membranes (Duo-Flux, F 60, Filtral) significantly reduced plasma B2M by 27-53%, indicating B2M elimination.
  • All dialyzers increased plasma elastase; cuprammonium cellulose plate showed the highest increase.

Conclusions:

  • High-permeability dialyzers can effectively eliminate plasma B2M.
  • Dialysis with conventional membranes does not lead to net generation or release of B2M.
  • Cuprammonium cellulose dialyzers induced a more pronounced increase in plasma elastase compared to others.