Anion exchange in type II pneumocytes freshly isolated from adult guinea-pig lung

We have studied chloride influx and efflux in a highly purified preparation of type II cells freshly isolated from adult guinea-pig lung using 36Cl-. Chloride uptake was time-dependent, saturable (Km < 10 mM) and was inhibited by 4,4'-diisothiocyanatostilbene-2,2'-disulphonic acid (DIDS; Ki approximately 80 microM). In the absence of external chloride (substituted by gluconate), 36Cl- uptake exhibited an overshoot above equilibrium. The rate of 36Cl- entry was strongly inhibited by addition of external nitrate; sulphate was a weaker inhibitor. 36Cl- efflux was stimulated by external bromide > bicarbonate > or = chloride > or = citrate; and was inhibited by propionate > acetate > oxalate. Although the "chloride channel blocker" 4-nitro-2-(3-phenylpropylamino)benzoate (0.14 mM) caused an inhibition, 36Cl- influx did not appear to be electrogenic. These data are compatible with the existence of a substantial electroneutral anion-exchange pathway for chloride transport in freshly isolated adult type II pneumocytes.