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Monocyte chemoattractant protein production in red cell incompatibility
R D Davenport1, M Burdick, R M Strieter
1Department of Pathology, University of Michigan Medical School, Ann Arbor.
Transfusion
|January 1, 1994
Summary
Monocyte chemoattractant protein-1 (MCP-1) is produced by leukocytes during red blood cell (RBC) incompatibility. This cytokine may enhance hemolytic transfusion reactions by recruiting monocytes.
Area of Science:
- Immunology
- Transfusion Medicine
Background:
- Mononuclear phagocytes are key players in hemolytic transfusion reactions.
- Monocyte chemoattractant protein-1 (MCP-1) is a cytokine specific for monocytes.
- Investigating MCP-1 production in red blood cell (RBC) incompatibility is crucial.
Purpose of the Study:
- To investigate the production of monocyte chemoattractant protein-1 (MCP-1) in response to red blood cell (RBC) incompatibility.
- To understand the role of MCP-1 in hemolytic transfusion reactions.
Main Methods:
- Incubation of whole blood with compatible or incompatible RBCs.
- Incubation of isolated mononuclear cells with anti-D-coated RBCs.
- Measurement of MCP-1 protein by ELISA and gene expression by Northern blot.
Main Results:
- Significant MCP-1 protein levels were detected 24 hours after adding incompatible RBCs.
- MCP-1 production was linked to increased MCP-1 mRNA levels.
- MCP-1 production occurred more rapidly in ABO than Rh incompatibility.
Conclusions:
- Peripheral blood leukocytes produce MCP-1 in response to RBC incompatibility.
- MCP-1 may recruit and activate monocytes, sustaining hemolytic transfusion reactions.
- MCP-1 plays a role in the positive feedback loop of these reactions.