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[Example of the use of affinity electrophoresis for the determiniation of antigens]
Comptes Rendus Hebdomadaires Des Seances De L'Academie Des Sciences. Serie D: Sciences Naturelles
|October 27, 1976
Abstract:
In this paper, affinity electrophoresis is employed to obtain a quantitative evaluation of an antigen using biospecific immunoadsorbant. As an example it is shown that human serum albumin reacts with its specific antibodies linked to agarose beads forming "rockets" in the gel. The height of these "rockets" corresponds to the quantity of albumin in the medium. This method assumes the immobilization of the antibodies in the gel with all the buffers employed.