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Related Experiment Videos

Cytokines decrease apolipoprotein accumulation in medium from Hep G2 cells

W H Ettinger1, V K Varma, M Sorci-Thomas

  • 1Department of Internal Medicine, Bowman Gray School of Medicine of Wake Forest University, Winston-Salem, NC.

Arteriosclerosis and Thrombosis : a Journal of Vascular Biology
|January 1, 1994
PubMed
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Cytokines, key inflammation mediators, lower cholesterol by reducing liver apolipoprotein synthesis and secretion. This study shows how inflammatory cytokines impact cholesterol metabolism and apolipoprotein levels.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Immunology

Background:

  • Cytokines are crucial mediators of inflammation.
  • Inflammation is associated with decreased plasma cholesterol levels (acquired hypocholesterolemia).
  • A potential mechanism involves reduced hepatic synthesis and secretion of apolipoproteins.

Purpose of the Study:

  • To investigate the effect of specific cytokines on apolipoprotein synthesis and secretion in liver cells.
  • To determine if cytokines influence apolipoprotein mRNA levels.
  • To explore the impact on cholesterol and triglyceride metabolism.

Main Methods:

  • Incubation of Hep G2 cells with tumor necrosis factor-alpha, interleukin-1 beta, and interleukin-6.
  • Measurement of apolipoprotein A-I, apolipoprotein B, and lecithin:cholesterol acyltransferase (LCAT) activity in the cell medium.

Related Experiment Videos

  • Analysis of cellular apolipoprotein A-I and apolipoprotein B mRNA concentrations.
  • Assessment of sterol synthesis and secretion rates using [14C]acetate incorporation.

    • Main Results:

    • Cytokines caused a dose-related decrease in apolipoprotein A-I, apolipoprotein B, and LCAT activity in the medium.
    • Cellular apolipoprotein A-I mRNA decreased, but apolipoprotein B mRNA levels were unaffected.
    • Triglyceride and cholesterol concentrations were reduced in the medium.
    • Interleukin-6 increased sterol synthesis but decreased sterol secretion.

    Conclusions:

    • These cytokines can reduce the hepatic synthesis and/or secretion of apolipoproteins.
    • This cytokine-induced reduction in apolipoproteins may contribute to acquired hypocholesterolemia during inflammation.
    • The findings provide insight into the molecular mechanisms linking inflammation and altered lipid metabolism.