[The effect of pro-oxidants on insulin secretion by the isolated rat pancreas]

The effect of pro-oxidants (tert-butyl peroxide and FeSO4) on rats perfusing isolated pancreas insulin secretion was studied. Pro-oxidants (10(-4) M) preperfusion during 20 minutes did not change significantly the basal insulin secretion and decreased glucose-stimulated secretion of the one. The content of pancreas TBA-active products increased twice after pro-oxidants effect and ratio of reduced and oxidized glutathiones liberation rates decreased from 2 to 0.5. The disturbance of hormone secretion as a result of decrease of Langerhans islets B-cells reduced glutathione pool is suppose to be one of the mechanisms of hypoinsulinemia development at host's states characterizing with lipids peroxidation activation.