Decreased teratogen susceptibility in mouse fetuses obtained from delayed mating

We examined whether the susceptibility of fetuses to a teratogen differs between fetuses from delayed mating and those from normal mating. Mitomycin-C (MMC; 2.5 mg/kg or 5.0 mg/kg) was administered to pregnant mice intraperitoneally on day 10 of gestation after either normal or delayed mating (6 h). The incidence of MMC-induced malformations in fetuses from delayed mating was significantly lower than in those from the normal mating group when the treatment time was adjusted to be at the same critical period according to the "catch-up" phenomenon of developmental progression in the delayed mating group.