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PCR amplification of microdissected wheat chromosome arms in a simple 'single tube' reaction
D Albani1, M J Côté, K C Armstrong
1Plant Research Centre, Agriculture Canada Research Branch, Ottawa, Ontario.
The Plant Journal : for Cell and Molecular Biology
|November 1, 1993
Abstract:
A novel method for the adaptor-mediated PCR amplification of microdissected chromosome arms is described. This simple and versatile protocol eliminates the need for enzymatic micromanipulation in nanoliter volumes and permits the efficient amplification of as little as two wheat chromosome arms in a 'single tube' reaction.