Dihydrotestosterone-sensitive neurons in the male rat ventromedial hypothalamus

Electrical stimulation of the midbrain central gray caused antidromic activation in 273 neurons in and around the hypothalamic ventromedial nucleus in 40 urethan-anesthetized male rats. The latency (range: 2-39 ms) and threshold (100-1,600 microA) of the responses were compared among intact males and orchidectomized males, which received either no treatment, daily injections of testosterone propionate or dihydrotestosterone, or a combined estradiol benzoate and progesterone injection. Recordings were from comparable neuronal pools, because probability distribution for the latency as well as histological localization of each response were not different among groups. Orchidectomy decreased the mean threshold, and either treatment reversed the effect. Dihydrotestosterone increased the mean threshold by reducing the number of cells with thresholds below 600 microA, to a level as in intact males. Estrogen reduced the number of cells responding at 300-900 microA, but at lower thresholds, fell short of replicating the distribution in intact males. Testosterone reinstated the distribution as in intact males. It was concluded that different subgroups of ventromedial hypothalamic neurons with specific sensitivity to metabolites of testosterone project to the central gray.