Related Experiment Video

Updated: Aug 7, 2026

Experimental Approaches to Study Mitochondrial Localization and Function of a Nuclear Cell Cycle Kinase, Cdk1
13:15

Experimental Approaches to Study Mitochondrial Localization and Function of a Nuclear Cell Cycle Kinase, Cdk1

Published on: February 25, 2016

Chromosomal mapping of human CDK2, CDK4, and CDK5 cell cycle kinase genes

D J Demetrick1, H Zhang, D H Beach

  • 1Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, NY 11724.

Cytogenetics and Cell Genetics
|January 1, 1994
PubMed

Insights

Cyclin-dependent kinases (CDKs) regulate cell division. This study mapped human CDK2, CDK4, and CDK5 genes, finding CDK2 and CDK4 at 12q13, a locus frequently altered in tumors, suggesting a link to cancer cell cycle dysregulation.

Area of Science:

  • Molecular Biology
  • Genetics
  • Cancer Research

Background:

  • Cyclin-dependent kinases (CDKs) are crucial regulators of the cell cycle.
  • Understanding the chromosomal locations of CDK genes is important for investigating their role in cell cycle control and disease.

Purpose of the Study:

  • To determine the chromosomal loci of human CDK2, CDK4, and CDK5 genes.
  • To investigate the potential association of these gene locations with chromosomal alterations in tumors.

Main Methods:

  • Genomic cloning of human CDK2, CDK4, and CDK5.
  • Fluorescence in situ hybridization (FISH) on human lymphocyte metaphase spreads for chromosomal mapping.

Main Results:

  • CDK2 and CDK4 genes were mapped to the same chromosomal locus, 12q13.
  • CDK5 gene was mapped to chromosome 7q36.
  • The 12q13 locus is known to be associated with chromosomal alterations in solid tumors, while 7q36 is not a major site of such alterations.

Conclusions:

  • The co-localization of CDK2 and CDK4 at 12q13, a frequently altered tumor locus, suggests their potential involvement in cancer development.
  • Alterations at the 12q13 locus in tumors may impact the regulation of CDK2 and CDK4, affecting cell cycle control.

More Related Videos

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis08:33

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay12:26

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Published on: May 3, 2018

Related Concept Videos

Positive Regulator Molecules01:45

Positive Regulator Molecules

To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
Positive Regulator Molecules02:39

Positive Regulator Molecules

Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Inhibition of CDK Activity02:34

Inhibition of CDK Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...