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Related Experiment Videos

Experimental toxoplasmosis in turkeys

J P Dubey1, M E Camargo, M D Ruff

  • 1Zoonotic Diseases Laboratory, U.S. Department of Agriculture, Beltsville, Maryland 20705.

The Journal of Parasitology
|December 1, 1993
PubMed
Summary

Turkeys infected with Toxoplasma gondii developed high antibody titers, detectable by modified agglutination test (MAT) and ELISA. T. gondii was isolated from various tissues, indicating systemic infection in turkeys.

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Area of Science:

  • Veterinary Parasitology
  • Immunology
  • Food Safety

Background:

  • Toxoplasma gondii is an important foodborne parasite with significant public health implications.
  • Turkeys are a potential reservoir for T. gondii, posing a risk for human consumption.
  • Understanding T. gondii infection dynamics in turkeys is crucial for developing effective control strategies.

Purpose of the Study:

  • To evaluate the susceptibility of turkeys to oral inoculation with T. gondii.
  • To assess the diagnostic sensitivity of different serological assays for detecting T. gondii antibodies in turkeys.
  • To determine the tissue distribution of T. gondii in experimentally infected turkeys.

Main Methods:

  • Oral inoculation of turkeys with varying doses of T. gondii oocysts (ME 49 strain).

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  • Clinical monitoring, necropsy, and parasite isolation from various tissues.
  • Serological testing using modified agglutination test (MAT), ELISA, latex agglutination, and indirect hemagglutination tests.

    • Main Results:

    • Turkeys inoculated with T. gondii developed high antibody titers detected by MAT and ELISA.
    • T. gondii was successfully isolated from heart, breast, and leg muscles, but not brain or liver.
    • ELISA and MAT demonstrated comparable sensitivity in detecting T. gondii antibodies, outperforming other tested assays.

    Conclusions:

    • Turkeys are susceptible to T. gondii infection, developing detectable antibody responses.
    • MAT and ELISA are reliable methods for diagnosing T. gondii infection in turkeys.
    • The study highlights the potential for T. gondii transmission through turkey meat products.