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Related Experiment Videos

Experimental specification of cell sorting, tissue spreading, and specific spatial patterning by quantitative

M S Steinberg1, M Takeichi

  • 1Department of Molecular Biology, Princeton University, NJ 08544.

Proceedings of the National Academy of Sciences of the United States of America
|January 4, 1994
PubMed
Summary

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Cellular sorting and arrangement depend on cell adhesion levels. Cells with higher P-cadherin expression form internal structures when mixed or outer layers when aggregated separately, confirming adhesion-driven morphogenesis.

Area of Science:

  • Developmental Biology
  • Cell Biology
  • Biophysics

Background:

  • Embryonic cell sorting and tissue patterning are complex processes.
  • Previous models suggested various factors influence cell arrangement, including chemotaxis and cell movement speed.
  • Mathematical predictions indicated cell adhesion levels alone could dictate segregation and spatial organization.

Purpose of the Study:

  • To test the hypothesis that differential expression of a single cell adhesion molecule determines cell sorting and tissue configuration.
  • To investigate the role of P-cadherin in mediating cell segregation and arrangement.

Main Methods:

  • Transfection of L cells with P-cadherin cDNA to create populations with varying expression levels.
  • Mixing intermixed cell populations and observing their segregation patterns.

Related Experiment Videos

  • Forming separate cell aggregates and observing their fusion and subsequent arrangement.

    • Main Results:

    • Intermixed cell populations segregated, forming a sphere-within-a-sphere structure with higher P-cadherin expressing cells in the core (medulla).
    • When initially separate aggregates fused, cells with higher P-cadherin expression were enveloped by the partner population (cortex).
    • These results demonstrate adhesion-based sorting and arrangement in the absence of other differential parameters.

    Conclusions:

    • Differential levels of cell adhesion, specifically P-cadherin expression, are sufficient to drive cell segregation and determine tissue architecture.
    • Morphogenetic movements and anatomical configurations can be precisely controlled by intercellular adhesive intensities.
    • The findings support the principle that cell adhesion is a primary determinant of tissue organization.