Tissue carriers for processing fragile bio-materials in immuno-electron microscopy

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Optimized Protocol for Retinal Wholemount Preparation for Imaging and Immunohistochemistry
08:32

Optimized Protocol for Retinal Wholemount Preparation for Imaging and Immunohistochemistry

Published on: December 13, 2013

Q Peng1, H P Richter

  • 1Department of Histology and Embryology, Tongji Medical University, Wuhan, P.R. China.

Acta Histochemica
|September 1, 1993
PubMed

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Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...