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Related Experiment Videos

Tissue carriers for processing fragile bio-materials in immuno-electron microscopy

Q Peng1, H P Richter

  • 1Department of Histology and Embryology, Tongji Medical University, Wuhan, P.R. China.

Acta Histochemica
|September 1, 1993
PubMed
Summary

New tissue carriers simplify processing fragile biological samples for electron microscopy. These devices ensure safe transport during critical steps like dehydration and embedding, enhancing immunogold techniques.

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Area of Science:

  • Materials Science
  • Biological Sciences
  • Microscopy Techniques

Background:

  • Processing fragile biological specimens for electron microscopy requires careful handling.
  • Conventional methods can risk sample damage during multiple processing steps.

Purpose of the Study:

  • To describe the preparation and utility of novel tissue carriers.
  • To facilitate the safe processing of biological materials from postfixation to embedding.

Main Methods:

  • Development of small, practical tissue carriers.
  • Utilizing carriers for vibratome sections through osmification, dehydration, and infiltration.
  • Embedding processed samples in epoxy resins within the carriers.

Main Results:

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  • Tissue carriers provide practical and safe transport for fragile samples.
  • Successful processing of samples through extended dehydration and infiltration steps.
  • Demonstrated suitability for post-embedding immunogold electron microscopy.

Conclusions:

  • The developed tissue carriers are effective tools for electron microscopy sample preparation.
  • These carriers enhance the reliability of processing delicate biological materials.
  • The method is particularly advantageous for immunogold labeling protocols.