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Cell abnormalities associated with human papillomavirus-induced squamous intraepithelial cervical lesions.
V Mayelo1, P Garaud, L Renjard
1Laboratoire de pathologie cellulaire, CHRU Bretonneau, Tours, France.
American Journal of Clinical Pathology
|January 1, 1994
Summary
This study found that specific cell abnormalities in Pap smears, like dyskaryotic cells and epithelial maturation changes, are reliable indicators for diagnosing human papillomavirus (HPV) infections and related cervical lesions.
Area of Science:
- Gynecologic Pathology
- Molecular Virology
- Cytopathology
Background:
- Cervical cancer screening relies on detecting cellular abnormalities in Papanicolaou (Pap) smears.
- Human papillomavirus (HPV) is the primary cause of cervical intraepithelial lesions.
- Accurate cytodiagnosis of HPV-associated lesions is crucial for effective management.
Purpose of the Study:
- To investigate the correlation between specific cytologic abnormalities in Pap smears and the presence of genital HPV genotypes.
- To identify the most reliable cellular indicators for diagnosing HPV-induced squamous intraepithelial cervical lesions.
Main Methods:
- Simultaneous analysis of 196 cervical scrapings for Papanicolaou smear cytology and HPV genotyping via polymerase chain reaction (PCR).
- Cytologic parameters included multinucleated squamous cells, dyskaryotic cells, koilocytes, and epithelial maturation changes.
- Multiple correspondence analysis was employed to assess the association between cytologic features and HPV presence.
Main Results:
- A significant association was observed between HPV infection and several cytologic abnormalities, including multinucleated squamous cells, dyskaryotic cells, koilocytes, and epithelial maturation changes.
- Squamous intraepithelial lesions showed a plurality of HPV-related cell abnormalities.
- Cellular changes related to epithelial maturation and dyskaryotic cells demonstrated high frequency and correlation with HPV infection.
Conclusions:
- Morphologically transformed keratinocytes (dyskaryotic cells) and cellular changes related to epithelial maturation are highly accurate and efficient cytologic markers for HPV-associated squamous intraepithelial cervical lesions.
- While koilocytes are indicative of HPV, their low detection rate suggests other markers are more reliable for cytodiagnosis.
- These findings enhance the cytodiagnostic accuracy for HPV-induced cervical lesions.