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Modification of epithelial permeability by cationic polypeptides

C J Tzan1, J R Berg, S A Lewis

  • 1Department of Physiology and Biophysics, University of Texas Medical Branch, Galveston 77555-0641.

The American Journal of Physiology
|December 1, 1993
PubMed
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Synthetic cationic polypeptides, similar to protamine sulfate, significantly increase urinary bladder apical membrane conductance. This effect is dependent on charge, voltage, and concentration, offering insights into bladder epithelial transport.

Area of Science:

  • Urology
  • Membrane Biophysics
  • Biochemistry

Background:

  • Protamine sulfate (PS), a cationic polypeptide rich in arginine, is known to increase mammalian urinary bladder apical membrane conductance.
  • The precise mechanism by which PS modulates bladder conductance remains incompletely understood, particularly the role of its cationic nature.

Purpose of the Study:

  • To investigate whether the observed effects of protamine sulfate on urinary bladder conductance are attributable to its cationic properties.
  • To characterize the influence of synthetic cationic polypeptides (CpP) on the electrical properties of the rabbit urinary bladder epithelium.

Main Methods:

  • Utilized synthetic cationic polypeptides (CpP), such as polyarginine, to study their effects on rabbit urinary bladder epithelial cells.
  • Measured changes in apical membrane cation and anion conductance in response to CpP application.

Related Experiment Videos

  • Investigated the voltage dependence, charge-related parameters, lanthanum inhibition, and self-inhibition effects of CpP on membrane conductance.

    • Main Results:

    • CpP induced a substantial increase in cation and anion conductance across the rabbit urinary bladder apical membrane.
    • The magnitude of conductance change was voltage-dependent.
    • Increased molecular charge and charge density of CpP accelerated the rate of conductance change.
    • Lanthanum ions (La3+) inhibited CpP's ability to alter membrane conductance.
    • Reversal rate of CpP-induced conductance depended on molecular charge and charge density.
    • Self-inhibition of CpP-induced conductance was inversely correlated with charge density and concentration-dependent.

    Conclusions:

    • The cationic nature of polypeptides, not solely protamine sulfate, is responsible for increasing urinary bladder apical membrane conductance.
    • CpP modulate bladder epithelial ion transport through mechanisms influenced by charge, density, voltage, and concentration.
    • These findings support a model explaining the effects of PS on urinary bladder conductive properties.