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Related Experiment Videos

Protein adsorption studies on 'standard' polymeric materials

J L Brash1, P Ten Hove

  • 1Department of Chemical Engineering and Pathology, McMaster University, Hamilton, Ontario, Canada.

Journal of Biomaterials Science. Polymer Edition
|January 1, 1993
PubMed
Summary

Protein adsorption to medical materials varies significantly. Cellulose showed minimal fibrinogen adsorption from plasma, unlike other materials which exhibited the Vroman effect, indicating complex surface interactions.

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Nonfouling biomaterials based on polyethylene oxide-containing amphiphilic triblock copolymers as surface modifying additives: adsorption of proteins from human plasma to copolymer/polyurethane blends.

Journal of biomedical materials research. Part A·2008

Area of Science:

  • Biomaterials Science
  • Surface Chemistry
  • Protein Adsorption

Background:

  • Understanding protein adsorption onto biomaterials is crucial for medical device design.
  • Fibrinogen adsorption influences biocompatibility and device performance.
  • The Vroman effect describes dynamic protein adsorption and displacement.

Purpose of the Study:

  • To quantify fibrinogen adsorption on different biomaterials (polydimethylsiloxane, polyethylene, polyvinylchloride, cellulose).
  • To compare protein adsorption from single protein solutions versus whole plasma.
  • To investigate the Vroman effect and its relation to material type and form (film vs. tubing).

Main Methods:

  • Studied adsorption of 125I-labelled fibrinogen from buffer and plasma.

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  • Materials tested: polydimethylsiloxane (PDMS), low-density polyethylene (PE), polyvinylchloride (PVC), and cellulose.
  • Tested materials in film and tubing forms, measuring adsorption at room temperature.

    • Main Results:

    • Single protein adsorption order: cellulose < PVC < PE = PDMS.
    • Plasma adsorption showed Vroman effect (maxima) on all surfaces except cellulose.
    • Cellulose exhibited very low fibrinogen adsorption from plasma.
    • Vroman effect was more pronounced on tubing than film samples.

    Conclusions:

    • Material type significantly impacts fibrinogen adsorption and Vroman effect behavior.
    • Cellulose demonstrates low fibrinogen affinity, suggesting potential for reduced thrombogenicity.
    • Surface form (film vs. tubing) influences adsorption dynamics and Vroman effect intensity.