Dramatic changes in control properties that accompany channelling and metabolite sequestration
Related Experiment Video
Updated: Nov 8, 2026

Cholinergic Ligand–dependent Modulation of Oxidative Phosphorylation Coupling in Digitonin-permeabilized BE(2)-C Neuroblastoma Cells
Published on: April 28, 2026
B N Kholodenko1, M Cascante, H V Westerhoff
1Department de Bioquimica i Fisiologia, Universitat de Barcelona, Spain.
Abstract:
A simple summation theorem describes the control of fluxes in 'ideal' metabolic pathways. This paper shows how this theorem and the control properties of a pathway change when direct transfer of intermediates and/or sequestration of metabolites involved in moiety conservations (by enzymes present at high concentrations) take place. The derived generalized summation theorem quantifies the extent to which metabolite sequestration decreases and direct metabolite transfer can increase the control exerted by enzymes on the flux. The implications of metabolite channelling for the control of fluxes are discussed quantitatively.
Related Concept Videos
Drug Biotransformation: Overview
Drug Biotransformation: Overview
Regulation of Metabolism
Drug Metabolism: Phase II Reactions
Phase II Conjugation Reactions: Overview
Hepatic Drug Excretion: Enterohepatic Cycling
Post-release drugs and metabolites can be reabsorbed into the body from the intestine. For conjugated metabolites like glucuronides, reabsorption requires enzymatic hydrolysis by intestinal microflora. This...

