Related Experiment Videos
Uninterrupted translation through putative 12-nucleotide coding gap in sequence of carA: business as usual
T M Tuohy1, T Kidd, R F Gesteland
1Department of Human Genetics, Eccles Institute of Human Genetics, University of Utah, Salt Lake City 84112-1107.
Journal of Bacteriology
|January 1, 1994
Abstract:
Previous work of others reported an untranslated stretch of 12 nucleotides in the 5' coding sequence of carA from Pseudomonas aeruginosa. However, N-terminal protein sequencing of carA-lacZ translational fusions shows that these 12 nucleotides are normally translated in a continuous triplet manner, both in P. aeruginosa and in Escherichia coli.