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[Morphological changes in the supporting cells of the utricular macula due to streptomycin intoxication]
1Department of Otolaryngology, Hiroshima University School of Medicine.
Abstract:
Morphological changes in cultured utricular supporting cells following streptomycin sulfate (SM) intoxication were investigated using an organ culture system. Utricles of guinea pig were exposed to 30 and 3mg/ml of SM for 1-3 days in culture. The number of lysosomes in the supporting cells increased daily, and mitochondria, myeloid bodies, granules and vesicles were observed within the lysosomes. As these components accumulated in the lysosomes, the number of granules and vesicles in the cytoplasma decreased. Acid phosphatase (AcPase) activity also decreased. After 1-3 days culture with SM, the culture medium was changed to a medium without SM. After removal of SM, the Golgi apparati appeared more developed and AcPase activity was higher. At the same time, lysosomes were markedly decreased in number and the endoplasmic reticulum showed a gradual reproduction. These findings suggest close relationships among the Golgi apparatus, lysosomes, secretory granules and the endplasmic reticulum.
Insights
Streptomycin sulfate (SM) intoxication causes cellular changes in cultured utricular supporting cells, increasing lysosomes and decreasing acid phosphatase activity. Recovery occurs after SM removal, with Golgi apparatus development and endoplasmic reticulum regeneration.
Area of Science:
- Ototoxicity research
- Cell biology
- Vestibular system science
Context:
- Investigating the cellular effects of aminoglycoside antibiotics, specifically streptomycin sulfate (SM).
- Utilizing an organ culture system of guinea pig utricles to model in vivo conditions.
- Examining morphological alterations in supporting cells under toxicant exposure.
Purpose:
- To elucidate the morphological changes in cultured utricular supporting cells induced by streptomycin sulfate (SM).
- To observe the dynamic cellular responses to SM intoxication and subsequent recovery.
- To understand the interrelationship between cellular organelles during SM exposure and withdrawal.
Summary:
- Exposure to SM led to increased lysosomes containing cellular debris and decreased acid phosphatase activity in supporting cells.
- Following SM removal, enhanced Golgi apparatus development and endoplasmic reticulum regeneration were observed.
- Lysosomal accumulation and subsequent decrease suggest a role in cellular debris clearance and recovery processes.
Impact:
- Provides insights into the cellular mechanisms of SM-induced ototoxicity.
- Highlights the potential for cellular recovery and organelle regeneration after toxicant removal.
- Suggests a functional link between the Golgi apparatus, lysosomes, and endoplasmic reticulum in cellular stress response.