Synaptic relationships between GABA-immunoreactive boutons and primary afferent terminals in the rat cuneate nucleus

The present study examined the synaptic relation between the primary afferent terminals and intrinsic neuronal elements in the rat cuneate nucleus. For this purpose, experimental degeneration after multiple cervicothoracic dorsal rhizotomies or anterograde transport of wheatgerm agglutinin conjugated to horseradish peroxidase were used to identify the primary afferent terminals, while immunogold postembedding staining was employed to identify the GABA-immunoreactive boutons. The combined procedure allowed us to demonstrate a direct synaptic relationship between the primary afferent terminals and GABA-immunoreactive boutons. At least two types of synaptic relation were observed between the primary afferent terminals, identified by their degenerating features or labeled by wheatgerm agglutinin conjugated to horseradish peroxidase, and the immunogold-labeled GABA-immunoreactive boutons (i) a GABA-immunoreactive bouton making a simple presynaptic contact with the primary afferent terminal; and (ii) a synaptic glomerular complex in which the centrally located primary afferent terminal was postsynaptic to a GABA-immunoreactive bouton and presynaptic to dendrites closely associated with it; both terminals were sometimes presynaptic to a common dendrite. It is speculated from this study that the incoming impulses from the forelimb area are modulated by the GABA-immunoreactive boutons in the cuneate nucleus of the rat.