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Enzyme-linked immunosorbent assay for antibodies to ZEBRA, an Epstein-Barr trans-activator
V Maréchal1, M C Meyohas, I Joab
1Laboratoire de Microbiologie, Hôpital Rothschild, Paris, France.
Research in Virology
|September 1, 1993
Abstract:
Patients with AIDS and AIDS-related complex often show symptoms of Epstein-Barr virus (EBV) reactivation. Several EBV-encoded trans-acting factors activate the EBV lytic cycle, and one, called ZEBRA (BamHI Z EBV replication activator), controls the switch of EBV from a latent to a productive cycle. We describe here a simple ELISA test using a bacterially expressed ZEBRA protein as antigen. Utilizing this technique, we evaluated the humoral response to ZEBRA antigen in 38 patients with nasopharyngeal carcinoma and 134 subjects with an asymptomatic HIV 1 infection. The control group consisted of 40 healthy adult blood donors.