[Development of a highly efficient method for separating bee venom components]

Factors preventing complete separation of melittin and phospholipase A2 under fractionation of the bee venom were studied. The methods of electrophoresis in PAAG, gel-filtration, ion-exchange and reverse-phase chromatography were used to show that the intermolecular interactions of suppositionally hydrophobic character prevent from complete separation of melittin and phospholipase A2. Conditions and methods are chosen which permit isolating melittin and phospholipase in a pure form.