Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Development of a highly efficient method for separating bee venom components]

G Iu Azhitskiĭ, D G Azhitskiĭ

    Ukrainskii Biokhimicheskii Zhurnal (1978)
    |July 1, 1993
    PubMed
    Summary

    Interactions between melittin and phospholipase A2 in bee venom hinder their separation. New methods were developed to successfully isolate both components in pure forms, advancing bee venom research.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    [Interaction between bee venom melittin and human blood albumin].

    Ukrainskii biokhimicheskii zhurnal (1978)·1995
    Same author

    [Effect of various specific agents on the heat stability of human serum albumin].

    Ukrainskii biokhimicheskii zhurnal (1978)·1993
    Same author

    [Qualitative changes in cerebrospinal fluid albumin in cerebral stroke].

    Zhurnal nevropatologii i psikhiatrii imeni S.S. Korsakova (Moscow, Russia : 1952)·1988
    Same author

    [A method of isoelectric focusing in a borate-polyol system with pH gradient of 4.0 to 5.6 in a thin layer of polyacrylamide gel].

    Voprosy meditsinskoi khimii·1987
    Same author

    [Isolation of influenza virus hemagglutinin by solubilization with the nonionic detergent O-14].

    Mikrobiologicheskii zhurnal·1987
    Same author

    [Two-dimensional electrophoresis of the products of limited hydrolysis of human serum albumin using isoelectric focusing in a borate-polyol system].

    Voprosy meditsinskoi khimii·1987

    Area of Science:

    • Biochemistry
    • Protein chemistry
    • Venom analysis

    Background:

    • Bee venom contains valuable bioactive components like melittin and phospholipase A2.
    • Complete separation of these components is crucial for their purification and subsequent applications.
    • Previous fractionation methods have faced challenges in achieving complete separation of melittin and phospholipase A2.

    Purpose of the Study:

    • To investigate the factors impeding the complete separation of melittin and phospholipase A2 during bee venom fractionation.
    • To identify and optimize conditions and methods for the pure isolation of melittin and phospholipase A2.

    Main Methods:

    • Polyacrylamide gel electrophoresis (PAAG)
    • Gel-filtration chromatography
    • Ion-exchange chromatography
    • Reverse-phase chromatography

    Main Results:

    • Intermolecular interactions, presumed to be hydrophobic in nature, were identified as the primary cause of incomplete separation.
    • Specific conditions and chromatographic techniques were successfully established.
    • These optimized methods enabled the isolation of both melittin and phospholipase A2 in high purity.

    Conclusions:

    • Hydrophobic interactions significantly contribute to the co-elution of melittin and phospholipase A2.
    • The developed fractionation strategies overcome these interactions, allowing for pure component isolation.
    • This research provides a foundation for obtaining pure melittin and phospholipase A2 for further study and application.

    Related Experiment Videos