Related Experiment Videos
[Isolating and enriching broths of Salmonellae]
Abstract:
The Authors have tested in 3 isolating and enriching broths some Salmonellae; the broths are available commercially (MUELLER-KAUFMANN broth, LEIFSON broth and HAJNA GN broth) and they tested their inhibitory capacity (concerning the growth of intestinal saprophyte flora) and their enriching capacities with regard to the various types of Salmonellae also. The method used consists in an exact evaluation of the increase percentage of the germs which were put to incubate in the broths at 37 degree C for 6, 15 and 24 hours. On completing the tests it is clearly evident that the MUELLER-KAUFMANN and the LEIFSON broths are far superior to the HAJNA broth (which proved to be not very activated in decreasing the growth of the intestinal saprophytes). At the same time, it should be pointed out the opportuneness of effecting the re-transplantation from the Salmonellae broths only after 24 hours, in order to permit a more abundant growth of the Salmonellae (and therefore easier and surer isolation) without, at the same time, observing an appreciable interference by the common intestinal saprophytes.