Comparison of HA-1A and E5 monoclonal antibodies to endotoxin in rats with endotoxaemia
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S J Boom1, J A Davidson, P Zhang
1Department of Surgery, Western Infirmary, Glasgow, UK.
Objective:
To investigate the ability of two anti-endotoxin IgM antibodies (10 mg/kg) to prevent the hyperdynamic response induced by infusion of Escherichia coli rough mutant endotoxin core types R1 and R2 in rats.
Design:
Randomised blind laboratory study.
Setting:
University department of surgery.
Material:
8 groups of 5 male Sprague-Dawley rats and 10 control animals.
Main Outcome Measure:
Percentage change in cardiac output during the period of observation.
Results:
E5 provided significant protection against R1 (p = 0.04) but not against R2. HA-1A gave significant protection against R2 (p < 0.04) but not against R1. In clinical isolates of E. coli the R1 type predominates (about 70%).
Conclusion:
These results may reflect true differences in specificity between the antibodies, but there remains the possibility that these are non-specific effects. Further controls with irrelevant murine and humanized IgM monoclonal antibodies are in progress.