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Swine interleukin 2 activity produced by mesenteric lymph node cells

H Iwata1, T Ueda, K Takayanagi

  • 1Department of Veterinary Hygiene, Faculty of Agriculture, Yamaguchi University, Japan.

The Journal of Veterinary Medical Science
|October 1, 1993
PubMed
Summary
This summary is machine-generated.

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This study details the optimal conditions for producing swine interleukin 2 (IL-2) using mesenteric lymph node cells and phytohemagglutinin-P. The research also characterized the physico-chemical properties of swine IL-2, finding similarities to other species.

Area of Science:

  • Immunology
  • Biochemistry

Background:

  • Interleukin 2 (IL-2) is a critical cytokine for immune responses.
  • Characterizing swine IL-2 is essential for understanding porcine immunology and developing related biotechnologies.

Purpose of the Study:

  • To establish optimal conditions for swine IL-2 production.
  • To characterize the physico-chemical properties of swine IL-2.

Main Methods:

  • Swine IL-2 activity was measured using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) colorimetric assay with the murine CTLL-2 cell line.
  • Mesenteric lymph node (MLN) cells were stimulated with phytohemagglutinin-P (PHA) under various conditions.
  • Partial purification was achieved using Sephacryl S-200 gel filtration.

Main Results:

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  • Maximal swine IL-2 activity was achieved with 1-2 x 10(7) MLN cells/ml and 20-40 µg/ml PHA for 48 hours.
  • Optimal stirring culture conditions involved 1 x 10(7) cells/ml, 20 µg/ml PHA, 200-400 ml scale, and 30 rpm for 20 hours.
  • The estimated molecular weight of swine IL-2 is ~32 kD, with a pI of ~5.3.
  • Swine IL-2 demonstrated sensitivity to acidic/alkaline pH, urea, trypsin, and heat (70°C).

Conclusions:

  • Optimal conditions for swine IL-2 production using MLN cells and PHA were identified.
  • The physico-chemical properties of swine IL-2 are comparable to those of human, murine, and feline IL-2.