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Replitase: a complex integrating dNTP synthesis and DNA replication

G P Reddy1, R S Fager

  • 1Cancer Center, University of Massachusetts Medical Center, Worcester 01605.

Critical Reviews in Eukaryotic Gene Expression
|January 1, 1993
PubMed
Summary
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Replitase, a mammalian cell complex, synthesizes deoxynucleoside triphosphates (dNTPs) and aids DNA replication. Its existence explains key cellular controls during DNA synthesis in the S phase.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • Deoxynucleoside triphosphates (dNTPs) are essential for DNA replication.
  • Mammalian cells possess complex enzymatic machinery for dNTP synthesis and DNA replication.

Purpose of the Study:

  • To present evidence for the existence of the replitase complex in mammalian cells.
  • To elucidate the role of replitase in dNTP synthesis and DNA replication during the S phase.

Main Methods:

  • Localization studies in intact and fractionated mammalian cells.
  • Precursor incorporation experiments in isolated complexes and permeabilized S phase cells.
  • Analysis of phenomena like channeling, compartmentation, and cross-inhibition.

Main Results:

Related Experiment Videos

  • Evidence supports the existence of a replitase complex in mammalian cells.
  • This complex is active only in the S phase and localized to the nuclear region.
  • Replitase provides a simple explanation for channeling, compartmentation, and cross-inhibition.

Conclusions:

  • Replitase is a crucial multienzyme complex for coordinated dNTP synthesis and DNA replication in mammalian cells.
  • The complex's S phase-specific nuclear localization ensures precise control over DNA synthesis.
  • The existence of replitase simplifies the understanding of fundamental cellular processes in DNA metabolism.